4J1U
Crystal structure of antibody 93F3 unstable variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.031, 112.571, 86.509 |
| Unit cell angles | 90.00, 115.28, 90.00 |
Refinement procedure
| Resolution | 78.330 - 2.580 |
| R-factor | 0.21306 |
| Rwork | 0.210 |
| R-free | 0.27564 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.706 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.071 | 0.523 |
| Number of reflections | 41988 | |
| <I/σ(I)> | 12.9 | 1 |
| Completeness [%] | 96.2 | 81.7 |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | 0.1M Tris, 18% PEG6000, VAPOR DIFFUSION, temperature 298K |
