4J09

Crystal Structure of LpxA bound to RJPXD33

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-26
Detector	Marmosaic 300
Wavelength(s)	0.97872
Spacegroup name	P 21 3
Unit cell lengths	96.399, 96.399, 96.399
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.460 - 1.900
R-factor	0.1723
Rwork	0.172
R-free	0.17710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.080
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER-TNT (BUSTER 2.10.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.200
High resolution limit [Å]	1.900
Number of reflections	23751
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293.15	1.4 M Potassium Phosphate, 33% DMSO, pH 6.5, VAPOR DIFFUSION, temperature 293.15K