4IZI
Crystal Structure of the Alpha1 dimer of Thermus thermophilus Transhydrogenase in P4(3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 43 |
| Unit cell lengths | 85.794, 85.794, 146.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.370 - 2.400 |
| R-factor | 0.20507 |
| Rwork | 0.202 |
| R-free | 0.25712 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l7d |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.473 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.370 | 2.462 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.049 | 0.469 |
| Number of reflections | 39243 | |
| <I/σ(I)> | 9.5 | 1.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | JCSG plus kit, condition A10: 0.2 M potassium formate, 20% w/v PEG3350, pH N.A., VAPOR DIFFUSION, temperature 293K |
