4IYS
Structural and ligand binding properties of the Bateman domain of human magnesium transporters CNNM2 and CNNM4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9723 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 55.754, 64.453, 53.953 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.667 - 1.800 |
| R-factor | 0.22 |
| Rwork | 0.218 |
| R-free | 0.26210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.297 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 18265 | |
| Completeness [%] | 96.5 | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 291.15 | 60mM NaH2PO4, 1.34M K2HPO4, pH 4.6, VAPOR DIFFUSION, temperature 291.15K |
