4IYI
The crystal structure of a secreted protein EsxB (wild-type, C-term. His-tagged) from Bacillus anthracis str. Sterne
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 31 |
| Unit cell lengths | 77.211, 77.211, 71.092 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.605 - 2.083 |
| R-factor | 0.155 |
| Rwork | 0.153 |
| R-free | 0.16680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 4IYH |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.875 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.120 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.136 | 0.616 |
| Number of reflections | 28219 | |
| <I/σ(I)> | 18.2 | 2.8 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.1M Sodium Acetate, 2.0M Ammonium Sulfate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
