4IY8
Bmlp3 - P21 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.930, 124.410, 67.890 |
| Unit cell angles | 90.00, 114.80, 90.00 |
Refinement procedure
| Resolution | 33.340 - 2.360 |
| R-factor | 0.17578 |
| Rwork | 0.173 |
| R-free | 0.25920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4efp |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.715 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.400 | 2.460 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.082 | 0.636 |
| Number of reflections | 35189 | |
| <I/σ(I)> | 15.7 | 2.74 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30% PEG MME 550, 0.1 M CaCl2, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
