4IY4
Structural and ligand binding properties of the Bateman domain of human magnesium transporters CNNM2 and CNNM4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 103.346, 103.346, 99.899 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.218 - 2.900 |
| R-factor | 0.2349 |
| Rwork | 0.232 |
| R-free | 0.28980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.722 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.218 | |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 12501 | |
| Completeness [%] | 99.9 | 99.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 291.15 | 2.8M AMMONIUM CHLORIDE, 4mM MANGANESE (II) CHLORIDE, 100mM SODIUM ACETATE , pH 4.6, VAPOR DIFFUSION, temperature 291.15K |
