4IY2
Structural and ligand binding properties of the Bateman domain of human magnesium transporters CNNM2 and CNNM4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 102.513, 104.593, 105.694 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.000 - 3.600 |
| R-factor | 0.29353 |
| Rwork | 0.293 |
| R-free | 0.29983 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.922 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.340 | 3.695 |
| High resolution limit [Å] | 3.600 | 3.602 |
| Number of reflections | 6355 | |
| Completeness [%] | 97.4 | 70 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291.15 | 250mM calcium acetate, 100mM imidazole , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
