4IX9
Crystal structure of subunit F of V-ATPase from S. cerevisiae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978836, 0.978683, 0.963626 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 47.146, 160.312, 102.429 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.530 - 2.330 |
| R-factor | 0.15523 |
| Rwork | 0.152 |
| R-free | 0.21154 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.792 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.410 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.084 | 0.434 |
| Number of reflections | 17018 | |
| <I/σ(I)> | 18.8 | 2.2 |
| Completeness [%] | 99.5 | 96.7 |
| Redundancy | 6.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 291 | 30% PEG 4000, 0.05M MgCl2,6H2O, 0.1M Tris HCl pH 8.8, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
