4IX5
Crystal structure of a Stt7 homolog from Micromonas algae in complex with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97854 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.860, 132.610, 55.180 |
| Unit cell angles | 90.00, 116.64, 90.00 |
Refinement procedure
| Resolution | 28.628 - 1.700 |
| R-factor | 0.1739 |
| Rwork | 0.172 |
| R-free | 0.20130 |
| Structure solution method | Difference Fourier |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.999 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.150 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.081 | 0.474 |
| Number of reflections | 75812 | |
| <I/σ(I)> | 14.8 | 4.1 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 100mM HEPES, 200mM MgCl2, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
