4IX4
Crystal structure of a Stt7 homolog from Micromonas algae in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97854 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.930, 133.027, 55.155 |
| Unit cell angles | 90.00, 116.48, 90.00 |
Refinement procedure
| Resolution | 39.641 - 1.499 |
| R-factor | 0.1587 |
| Rwork | 0.157 |
| R-free | 0.19880 |
| Structure solution method | Difference Fourier |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.019 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.499 | 1.500 |
| Number of reflections | 110828 | |
| <I/σ(I)> | 32.88 | 4.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 100mM HEPES, 200mM MgCl2, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
