4IWK
Crystal structure of iron soaked (overnight) ferritin from Pseudo-nitzschia multiseries
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 126.251, 126.251, 170.299 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.950 - 1.650 |
| R-factor | 0.1772 |
| Rwork | 0.176 |
| R-free | 0.20460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.493 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
| Rmerge | 0.065 | 0.046 | 0.507 |
| Number of reflections | 164004 | ||
| <I/σ(I)> | 16.8 | ||
| Completeness [%] | 99.6 | 97.9 | 100 |
| Redundancy | 10.7 | 16.6 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | pH 5.5, VAPOR DIFFUSION, HANGING DROP |
