4IWG
Crystal Structure of the Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii (in C2221 form)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2011-09-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97921, 0.97938, 0.96414 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.209, 172.939, 147.422 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.978 - 2.472 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.23670 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.085 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.560 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.053 | 0.460 |
| Number of reflections | 37448 | |
| <I/σ(I)> | 4.6 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 0.1M (NH4)2SO4, 0.3M sodium formate, 0.1M sodium acetate, 3% PGA-LM, 20% MPD , pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
