4IVJ
Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, triple mutant, I222 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-08-29 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.542 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 129.670, 160.980, 167.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.419 - 7.351 |
| R-factor | 0.2771 |
| Rwork | 0.276 |
| R-free | 0.28760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1BRO and 1AA7 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.390 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | |
| Low resolution limit [Å] | 29.283 | 32.870 |
| High resolution limit [Å] | 7.350 | 23.240 |
| Rmerge | 0.082 | 0.043 |
| Number of reflections | 2438 | 34 |
| <I/σ(I)> | 13.24 | 26.34 |
| Completeness [%] | 95.2 | 54.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | 0.1M Na Citrate pH 4.4, 10% PEG 3000, vapor diffusion, hanging drop, temperature 298K |
