4IVJ

Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, triple mutant, I222 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-08-29
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.542
Spacegroup name	I 2 2 2
Unit cell lengths	129.670, 160.980, 167.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.419 - 7.351
R-factor	0.2771
Rwork	0.276
R-free	0.28760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1BRO and 1AA7
RMSD bond length	0.015
RMSD bond angle	2.390
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.1)
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell
Low resolution limit [Å]	29.283	32.870
High resolution limit [Å]	7.350	23.240
Rmerge	0.082	0.043
Number of reflections	2438	34
<I/σ(I)>	13.24	26.34
Completeness [%]	95.2	54.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	298	0.1M Na Citrate pH 4.4, 10% PEG 3000, vapor diffusion, hanging drop, temperature 298K