4IUS
GCN5-related N-acetyltransferase from Kribbella flavida.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.993, 55.812, 104.702 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.500 - 1.300 |
| R-factor | 0.1295 |
| Rwork | 0.128 |
| R-free | 0.15560 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.638 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 32.500 | 50.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 3.530 | 1.300 |
| Rmerge | 0.066 | 0.040 | 0.379 |
| Number of reflections | 57044 | ||
| <I/σ(I)> | 14.4 | 2.23 | |
| Completeness [%] | 97.6 | 96.8 | 75.6 |
| Redundancy | 4.7 | 4.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 2 M ammonium sulfate, 0.1 M Tris-HCl, 0.1 M sodium malonate , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
