4IUJ
Structure of Polymerase acid protein (PA) from Influenzavirus A Influenza A virus A, WILSON-SMITH/1933 (H1N1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 68.800, 68.800, 395.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.600 - 1.900 |
| R-factor | 0.1751 |
| Rwork | 0.174 |
| R-free | 0.20140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2znl |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.334 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
| Rmerge | 0.072 | 0.052 | 0.520 |
| Number of reflections | 45117 | 586 | 3263 |
| <I/σ(I)> | 19.09 | 32.09 | 4.54 |
| Completeness [%] | 99.0 | 84.8 | 100 |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | Molecular Dimensions Morpheus screen g50: 10% PEG 8000, 20% ethylene glycol; 20mM of each Na-formate, Ammonium-acetate, Na3-citrate, NaK D/L tartrate, Na-oxamate; 100mM MOPS/HEPES pH 7.5; INVAN.07057.A.D15.PD909136 AT 20.45MG/ML, direct cryo, vapor diffusion, sitting drop, temperature 290K |
