4IUG

Crystal structure of beta-galactosidase from Aspergillus oryzae in complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97625
Spacegroup name	P 32 2 1
Unit cell lengths	146.380, 146.380, 136.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.914 - 2.600
R-factor	0.1821
Rwork	0.180
R-free	0.21380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tg7
RMSD bond length	0.009
RMSD bond angle	1.328
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.914	2.700
High resolution limit [Å]	2.600	2.600
Number of reflections	52147
Completeness [%]	99.8	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	295	0.1 M cadmium chloride, 0.1 M sodium acetate, 30 % (w/v) PEG 400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K