4ITZ
Crystal structure of the FK506 binding domain of Plasmodium vivax FKBP35 in complex with a tetrapeptide substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-11-02 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.123, 45.798, 54.786 |
| Unit cell angles | 90.00, 112.47, 90.00 |
Refinement procedure
| Resolution | 29.981 - 1.650 |
| R-factor | 0.1752 |
| Rwork | 0.174 |
| R-free | 0.20690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.458 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.500 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.052 | 0.347 |
| Number of reflections | 27234 | |
| <I/σ(I)> | 25.4 | 5 |
| Completeness [%] | 92.7 | 88.8 |
| Redundancy | 10.7 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 2.85M Ammonium aulphate, 0.1M MES buffer pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
