4IT6
Crystal structure of amino acid residues 1-120 of CG17282
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97243, 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.503, 46.461, 69.146 |
| Unit cell angles | 90.00, 105.64, 90.00 |
Refinement procedure
| Resolution | 30.412 - 1.900 |
| R-factor | 0.2629 |
| Rwork | 0.261 |
| R-free | 0.27960 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.096 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.114 | 0.076 | 0.499 |
| Number of reflections | 20495 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 96.2 | 99.6 | 74.5 |
| Redundancy | 6.3 | 7.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293 | 4M sodium formate pH 6.9, 0.1 M Na-cacodylate pH 6.5 and 1% (w/v) polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
