4ISM
Crystal structure of ferritin from Pseudo-nitzschia multiseries soaked with zinc
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 175.112, 175.112, 175.112 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.510 - 2.000 |
| R-factor | 0.1861 |
| Rwork | 0.184 |
| R-free | 0.23150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.940 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.086 | 0.040 | 0.452 |
| Number of reflections | 120229 | ||
| <I/σ(I)> | 14.2 | ||
| Completeness [%] | 99.9 | 99.5 | 100 |
| Redundancy | 14.7 | 14.4 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
