4ISC

Crystal structure of a putative Methyltransferase from Pseudomonas syringae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97931
Spacegroup name	P 43 21 2
Unit cell lengths	43.460, 43.460, 181.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.260 - 2.780
R-factor	0.19439
Rwork	0.190
R-free	0.27744
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.733
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.260	2.780
High resolution limit [Å]	2.710	2.710
Rmerge	0.069
Number of reflections	5263
<I/σ(I)>	24.3	3.8
Completeness [%]	99.4	99.3
Redundancy	7.6	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2 M Na acetate, 0.1 M Bis Tris propane, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K