4IRY
Influenza A virus tail-loop free nucleoprotein at 2.8 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9179 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.183, 155.618, 97.774 |
| Unit cell angles | 90.00, 90.94, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| Rwork | 0.241 |
| R-free | 0.29780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iqh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.115 | 0.427 |
| Number of reflections | 21286 | |
| <I/σ(I)> | 9.1 | 2.5 |
| Completeness [%] | 97.5 | 79 |
| Redundancy | 4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100mM Tris pH8.5, 18% PEG3350, 10% Glycerol, 10mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
