4IRM

Crystal structure of mntc r116a mutant exhibits flexibility in the c-terminal domain

Replaces:  3V63

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 31 2 1
Unit cell lengths	128.400, 128.400, 90.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.500 - 3.500
R-factor	0.294
Rwork	0.294
R-free	0.30500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ujp
RMSD bond length	0.050
RMSD bond angle	2.950
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.500	3.690
High resolution limit [Å]	3.500	3.500
Rmerge	0.078
Number of reflections	11028
<I/σ(I)>	15	6.2
Completeness [%]	99.0
Redundancy	5.3	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	290	PEG 4000, SODIUM CACODYLATE, 50mM ZINC CHLORIDE,, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K