4IRI
Auto-inhibited ERG Ets Domain-DNA Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-11-03 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 51.486, 51.486, 141.313 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.760 - 2.770 |
| R-factor | 0.19144 |
| Rwork | 0.191 |
| R-free | 0.20994 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.636 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.760 | 2.850 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Number of reflections | 5250 | |
| Completeness [%] | 99.1 | 94.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.2M ammonium chloride, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
