4IR7
Crystal Structure of Mtb FadD10 in Complex with Dodecanoyl-AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-01 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 138.212, 138.212, 82.582 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.034 - 2.800 |
| R-factor | 0.2286 |
| Rwork | 0.226 |
| R-free | 0.27950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.604 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 5025.000 | 2.890 |
| High resolution limit [Å] | 2.800 | 4017.000 | 2.750 |
| Rmerge | 0.109 | 0.076 | 0.890 |
| Number of reflections | 24707 | ||
| <I/σ(I)> | 6.7 | ||
| Completeness [%] | 99.9 | 100 | 100 |
| Redundancy | 7.2 | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | PEG 6000, LiSO4, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
