4IR1
Polymerase-DNA Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.750, 57.250, 111.301 |
| Unit cell angles | 90.00, 90.44, 90.00 |
Refinement procedure
| Resolution | 47.660 - 2.380 |
| R-factor | 0.2171 |
| Rwork | 0.214 |
| R-free | 0.26934 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.565 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.510 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.514 | |
| Number of reflections | 44007 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 15% MPD , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
