4IQN

Crystal structure of uncharacterized protein from Salmonella enterica subsp. enterica serovar typhimurium str. 14028s

Replaces:  4HG1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97931
Spacegroup name	P 1 21 1
Unit cell lengths	62.138, 95.477, 69.897
Unit cell angles	90.00, 109.66, 90.00

Refinement procedure

Resolution	31.130 - 1.750
R-factor	0.13799
Rwork	0.135
R-free	0.19568
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.548
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MLPHARE
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.088	0.623
Number of reflections	76983
<I/σ(I)>	41.9	2.71
Completeness [%]	99.8	97.8
Redundancy	7.1	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	297	0.1M HEPES, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K