4IQH

Crystal Structure Analysis of Dysferlin C2A variant 1 (C2Av1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	90
Detector technology	CCD
Collection date	2012-01-01
Detector	Mercury 3
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	71.160, 71.160, 137.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.764
R-factor	0.1945
Rwork	0.192
R-free	0.21530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ihb
RMSD bond length	0.024
RMSD bond angle	1.816
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.760
Number of reflections	36605
Completeness [%]	95.7
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	280	16% PEG 20000, 0.1M sodium citrate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 280K
1	VAPOR DIFFUSION, HANGING DROP	6.2	280	16% PEG 20000, 0.1M sodium citrate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 280K