4IPU
Crystal structure of Pseudomonas aeruginosa (strain: PAO1) type IV minor pilin FimU in space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.982, 37.246, 103.123 |
| Unit cell angles | 90.00, 105.33, 90.00 |
Refinement procedure
| Resolution | 49.726 - 1.351 |
| R-factor | 0.1768 |
| Rwork | 0.176 |
| R-free | 0.20270 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.052 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.065 | 0.424 |
| Number of reflections | 99928 | |
| <I/σ(I)> | 20.5 | 3.17 |
| Completeness [%] | 99.8 | 96.9 |
| Redundancy | 6.3 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 0.2 M ammonium formate, 20% (w/v) PEG 3350, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
