4IPG

Structure of the N-terminal domain of RPA70, E7R, E100R mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-05-22
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	38.490, 53.790, 54.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.266 - 1.580
R-factor	0.1634
Rwork	0.162
R-free	0.20070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b29
RMSD bond length	0.018
RMSD bond angle	1.796
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.266	1.679
High resolution limit [Å]	1.580	1.580
Number of reflections	16007
<I/σ(I)>	18.3
Completeness [%]	99.9	100
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	294	100 mM Citrate, 200 mM NaCl, 30% PEG 8000, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K