4IP6
C-terminal domain of the thiol:disulfide interchange protein DsbD, Q488A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-12-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.891, 51.507, 84.299 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.620 - 2.230 |
| R-factor | 0.1865 |
| Rwork | 0.183 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fwf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.938 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.310 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.155 | 0.382 |
| Number of reflections | 7224 | |
| <I/σ(I)> | 7.5 | 1.96 |
| Completeness [%] | 91.7 | 71.2 |
| Redundancy | 3.3 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 21 mg/mL protein, 25% PEG4000, 0.1 M sodium cacodylate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
