4IP4

Crystal structure of l-fuconate dehydratase from Silicibacter sp. tm1040 liganded with Mg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-08-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	101.051, 141.332, 59.778
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.588 - 2.128
R-factor	0.1425
Rwork	0.140
R-free	0.18340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hxt
RMSD bond length	0.007
RMSD bond angle	1.051
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	38.588
High resolution limit [Å]	2.128
Number of reflections	48729
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	25% PEG 3350, 0.1M Hepes, 0.2M Magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K