4IOY

Structure of the Spt16 Middle Domain Reveals Functional Features of the Histone Chaperone FACT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-01-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 65
Unit cell lengths	133.978, 133.978, 40.342
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.690 - 1.945
R-factor	0.1774
Rwork	0.177
R-free	0.19660
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.086
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.13)
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.020
High resolution limit [Å]	1.945	4.200	1.945
Rmerge	0.078	0.047	0.517
Number of reflections	29648
<I/σ(I)>	17.4
Completeness [%]	96.5	100	84.5
Redundancy	8.6	11.6	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277.15	0.1 M sodium/potassium phosphate, pH 6.0, 0.2 M sodium chloride, 40% PEG400, 10 mM TCEP-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K
1	VAPOR DIFFUSION, HANGING DROP	6	277.15	0.1 M sodium/potassium phosphate, pH 6.0, 0.2 M sodium chloride, 40% PEG400, 10 mM TCEP-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K