4IOB
Crystal structure of the GGDEF domain of PA1120 (YfiN or TpbB) from Pseudomonas aeruginosa at 2.7 Ang.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 70.351, 70.351, 106.866 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.780 |
| R-factor | 0.27841 |
| Rwork | 0.278 |
| R-free | 0.27978 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wb4 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.460 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.940 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.095 | 0.607 |
| Number of reflections | 4343 | |
| <I/σ(I)> | 31.11 | 3.3 |
| Completeness [%] | 99.6 | |
| Redundancy | 13.7 | 9.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 294 | 300nl+300nl of 3.7 mg/ml protein solution (100 mM NaCl, 10 mM Tris pH=8.0, 2% glycerol) and 0.1 M Sodium Citrate dehydrate pH 5.6, 35% v/v tert-butanol., VAPOR DIFFUSION, SITTING DROP, temperature 294K |
