4IO5
Crystal Structure of the AvGluR1 ligand binding domain complex with alanine at 1.72 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.428, 100.691, 56.655 |
| Unit cell angles | 90.00, 116.44, 90.00 |
Refinement procedure
| Resolution | 29.510 - 1.721 |
| R-factor | 0.1597 |
| Rwork | 0.159 |
| R-free | 0.19480 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4io2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.275 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: dev_1039)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.750 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.061 | 0.570 |
| Number of reflections | 58814 | |
| <I/σ(I)> | 23.1 | 2.5 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 17.5% PEG 3350, 0.1 M NaCitrate, 0.1 M BisTris Propane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
