4IO2
Crystal Structure of the AvGluR1 ligand binding domain complex with glutamate at 1.37 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.370, 101.052, 56.744 |
| Unit cell angles | 90.00, 116.46, 90.00 |
Refinement procedure
| Resolution | 29.511 - 1.370 |
| R-factor | 0.1381 |
| Rwork | 0.137 |
| R-free | 0.16880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s50 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.474 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1039)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.390 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.043 | 0.590 |
| Number of reflections | 116416 | |
| <I/σ(I)> | 26.6 | 2 |
| Completeness [%] | 97.7 | 95.2 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 3350, 0.1 M NaCitrate, 0.1 M BisTris Propane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
