4INL
Crystal structure of SplD protease from Staphylococcus aureus at 2.1 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-16 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 174.273, 174.273, 174.273 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.410 - 2.100 |
| R-factor | 0.21391 |
| Rwork | 0.212 |
| R-free | 0.25578 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w7s |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.529 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.270 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.076 | 0.159 |
| Number of reflections | 11885 | |
| <I/σ(I)> | 11 | 3.1 |
| Completeness [%] | 86.7 | 72.1 |
| Redundancy | 3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 0.1 M HEPES, pH 7.0, 30% v/v Jeffamine ED-2001, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
