4INC
Human Histidine Triad Nucleotide Binding Protein 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.826, 74.952, 77.796 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.018 - 1.190 |
| R-factor | 0.1608 |
| Rwork | 0.160 |
| R-free | 0.17810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1av5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.270 |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 77.796 | 77.796 | 1.250 |
| High resolution limit [Å] | 1.190 | 3.760 | 1.190 |
| Rmerge | 0.051 | 0.032 | 0.320 |
| Total number of observations | 14423 | 45291 | |
| Number of reflections | 71181 | ||
| <I/σ(I)> | 19.2 | 18.1 | 2.1 |
| Completeness [%] | 99.2 | 99.6 | 95.5 |
| Redundancy | 6.1 | 5.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM MES, 40% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
