4IN5
(M)L214G mutant of the Rhodobacter sphaeroides Reaction Center
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-08-19 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 139.109, 139.109, 184.692 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 55.620 - 2.200 |
R-factor | 0.18249 |
Rwork | 0.181 |
R-free | 0.20619 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j8c |
RMSD bond length | 0.017 |
RMSD bond angle | 2.633 |
Data scaling software | SCALA (3.3.16) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 120.472 | 55.558 | 2.320 |
High resolution limit [Å] | 2.200 | 6.960 | 2.200 |
Rmerge | 0.035 | 0.590 | |
Number of reflections | 105008 | ||
<I/σ(I)> | 11.3 | 14.8 | 1.2 |
Completeness [%] | 99.9 | 98.3 | 100 |
Redundancy | 5.5 | 5.2 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 1 M potassium phosphate, 3.5% 1,2,3-heptanetriol, and 0.1% LDAO precipitant solution, 1.52 M potassium phosphate reservoir solution, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |