4IN3
Crystal Structure of the Chs5-Bch1 Exomer Cargo Adaptor Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.391, 155.686, 99.054 |
| Unit cell angles | 90.00, 95.34, 90.00 |
Refinement procedure
| Resolution | 38.730 - 2.936 |
| R-factor | 0.2396 |
| Rwork | 0.237 |
| R-free | 0.28890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4GNS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.907 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 7.860 | 2.900 |
| Number of reflections | 43750 | ||
| <I/σ(I)> | 8.59 | 2.51 | |
| Completeness [%] | 96.0 | 94.2 | 68.7 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | 15% PEG8000, 19% glycerol, 0.1 M Tris, pH 8.0, 0.2 M sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
