4IMV
Ricin A-chain variant 1-33/44-198 with engineered disulfide bond, R48C/T77C/D75N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2012-06-18 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 51.585, 72.336, 94.694 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.480 - 2.250 |
| R-factor | 0.2156 |
| Rwork | 0.214 |
| R-free | 0.24229 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.369 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.480 | 2.340 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 8640 | |
| <I/σ(I)> | 13.01 | 3.62 |
| Completeness [%] | 99.1 | 91.6 |
| Redundancy | 6.88 | 4.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 290 | 0.17 M Ammonium Sulfate, 25.5% (w/v) Peg 4000, 15% Glycerol , pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
