4IMM

The crystal structure of BamB from Moraxella catarrhalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	77
Detector technology	PIXEL
Collection date	2012-03-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	65.300, 78.620, 70.990
Unit cell angles	90.00, 92.11, 90.00

Refinement procedure

Resolution	41.544 - 2.330
R-factor	0.1916
Rwork	0.189
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.087
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.544	2.450
High resolution limit [Å]	2.330	2.330
Rmerge	0.097
<I/σ(I)>	15
Completeness [%]	99.9	99
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	1M MES, pH6.5 1M Imidazole 0.03M Magnesium chloride 0.03M Calcium chloride 32% PEG 3000, VAPOR DIFFUSION, HANGING DROP, temperature 291K