4IMH
Crystal Structure of Cytoplasmic Heme Binding Protein, PhuS, from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.09 |
| Spacegroup name | I 4 |
| Unit cell lengths | 187.730, 187.730, 42.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.978 - 1.976 |
| R-factor | 0.1777 |
| Rwork | 0.175 |
| R-free | 0.22150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2HQ2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.452 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.930 | 2.080 |
| High resolution limit [Å] | 1.976 | 1.976 |
| Rmerge | 0.059 | 0.240 |
| Number of reflections | 51833 | |
| <I/σ(I)> | 15.7 | 5.6 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.2M MgCl2, 0.1M HEPES(7.5), 25% polyacrylic acid 5100, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
