4IMC
Crystal Structure of Pasteurella multocida N-Acetyl-D-Neuraminic acid lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.946, 113.471, 82.238 |
| Unit cell angles | 90.00, 111.35, 90.00 |
Refinement procedure
| Resolution | 113.470 - 1.850 |
| R-factor | 0.1742 |
| Rwork | 0.172 |
| R-free | 0.20890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.353 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 113.471 |
| High resolution limit [Å] | 1.850 |
| Rmerge | 0.055 |
| Number of reflections | 112841 |
| <I/σ(I)> | 9.3 |
| Completeness [%] | 95.8 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 21% PEG1000, 0.15 M sodium chloride, 0.1 M sodium potassium phosphate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
