4IM9
Cystal structure of DnaG primase C-terminal domain from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-05-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.412, 73.779, 132.653 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.326 - 2.460 |
| R-factor | 0.22885 |
| Rwork | 0.227 |
| R-free | 0.26369 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.552 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.326 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.074 | 0.404 |
| Number of reflections | 25046 | |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 99.9 | 98.3 |
| Redundancy | 4.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.1 M Sodium cacodylate trihydrate (pH 6.5), 0.2 M MgCl2, 30% W/v PEG 3350 , VAPOR DIFFUSION, HANGING DROP, temperature 289K |
