4IM8
low resolution crystal structure of mouse RAGE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1. |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 115.810, 115.810, 115.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.162 - 3.503 |
| R-factor | 0.2624 |
| Rwork | 0.258 |
| R-free | 0.33610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cjj |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.756 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 3672 | |
| <I/σ(I)> | 8.1 | 4 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 13.1 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M Tris pH 8.5, 200mM MgCl2 and 22% PEG 400. Protein was purified on gel filtration in the presence of excess dodecasaccharide heparan sulfate that caused a shift in the elution profile from monomer to hexamer, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
