4ILL
Recognition and Cleavage of a non-structured CRISPR RNA by its Processing Endoribonuclease Cas6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-04-21 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.735, 68.542, 79.831 |
| Unit cell angles | 90.00, 119.12, 90.00 |
Refinement procedure
| Resolution | 32.000 - 2.484 |
| R-factor | 0.2071 |
| Rwork | 0.204 |
| R-free | 0.23860 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.067 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 2.600 |
| High resolution limit [Å] | 2.480 | 2.500 |
| Number of reflections | 24962 | |
| <I/σ(I)> | 41.4 | |
| Completeness [%] | 93.6 | 54.5 |
| Redundancy | 9.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 303 | 200mM MgCl2, 100mM Tris-Cl (pH 7.6), 32% PEG 400. , VAPOR DIFFUSION, HANGING DROP, temperature 303K |
