4ILI
Crystal structure of an Aar2p S253E phosphomimetic mutant protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-13 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 62.820, 62.820, 326.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.259 - 3.205 |
| R-factor | 0.2627 |
| Rwork | 0.260 |
| R-free | 0.30540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sbt |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.584 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.290 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.113 | 0.915 |
| Number of reflections | 13092 | |
| <I/σ(I)> | 14 | 2 |
| Completeness [%] | 99.5 | 98.6 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.75 | 298 | 40 mM imidazole, pH 7.75, 0.95 M sodium potassium tartrate and 0.2 M sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
