4ILA
The pentameric ligand-gated ion channel GLIC A237F in complex with Cesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 2.066 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.404, 133.232, 160.177 |
| Unit cell angles | 90.00, 102.50, 90.00 |
Refinement procedure
| Resolution | 19.980 - 3.500 |
| R-factor | 0.1908 |
| Rwork | 0.190 |
| R-free | 0.20820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.125 | 0.492 |
| Number of reflections | 47035 | |
| <I/σ(I)> | 6.9 | 2.4 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 290 | 12-15% PEG 4K, 0.1M Na Acetate pH 4, 0.2M NaSCN, 0.2M CsCl, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
