4IL9
The pentameric ligand-gated ion channel GLIC A237F in complex with bromide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9193 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.520, 132.140, 159.960 |
| Unit cell angles | 90.00, 102.16, 90.00 |
Refinement procedure
| Resolution | 41.770 - 2.830 |
| R-factor | 0.202 |
| Rwork | 0.201 |
| R-free | 0.21410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hfi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.800 | 3.000 |
| High resolution limit [Å] | 2.830 | 2.850 |
| Rmerge | 0.081 | 0.593 |
| Number of reflections | 85622 | |
| <I/σ(I)> | 12.5 | 2.4 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 290 | 12-15% PEG 4K, 0.1M Na Acetate pH 4, 0.2M NaSCN, 0.2M NaBr, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
